Bio-rad Bio-Beads S-X Media Manual do Utilizador

Bio-Beads®S-X BeadsGel Permeation ChromatographyInstruction ManualLIT263C 9/2/98 1:13 PM Page A

position. Then, displace the tetrahydrofuran with 3 bed volumesof chloroform.Flow RateBio-Beads S-X beads have the capacity for different flowrates, d

matogram to establish the time periods to use when collecting aspecific fraction.RegenerationRegeneration of Bio-Beads S-X beads may be necessary ifco

ApplicationsHarmon reviewed the many different types of gels that areavailable to the chromatographer, and reported that the relative-ly soft gels, su

analysis of fish lipid extracts,31and for fractionation of foodgrade poly (vinyl chloride) resin.32, 33A variety of lipophilic polymer substances has

1).Fig. 1. Separation of triglycerides and hydrocarbons onBio-Beads S-X1 and S-X2 beads in benzene (two beds inseries). 1 = tristearin; 2 = trimyriste

Bio-Beads S-X2 and S-X8 beads have been used in multi-column system for separating various components of tall oil,wood resin, and gum resin by gel per

Ault and Spurgeon used Bio-Beads S-X3 beads for specificseparation of chlorinated pesticides form animal fat.34Aitzetmuller has used Bio-Beads S-X bea

ReferencesReference Application1. Harmon, D. J., Sep. Sci., 5, 403 (1970). Bead swelling invarious solvents2. Stalling, D. L., et al., J.A.O.A.C., 55,

Reference Application12. Hood, C. I., Schoen, F. J., Coleman, S. E. and Tissue reactionMickley, L. D., J. Biomed. Materials Res., 18,1031 (1984).13. S

Reference Application23. Henfrickson, J. G., J. Chromatog., 32, 543 Hydrocarbons(1968).24. Cantow, M. J. R., et al., J. Polymer Sci., 5, 987 MW deter

Table of ContentsPageIntroduction... 1Technical Description ...

Product InformationSwollenM.W. M.W. Bed Vol.Catalog Product Mesh Exclusion Operating ml/gNumber Description Size Limit Range Benzene152-2150 Bio-Beads

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT263 Rev CLIT263C 9/2/98 1:14 PM Page 19

1IntroductionBio-Beads S-X beads are a series of porous crosslinkedpolystyrene polymers used for gel permeation separations oflipophilic polymers and

2of the matrix is achieved with relatively nonpolar, aromaticsolvents. The beads are typically used with benzene,toluene, xylene, carbon tetrachloride

3Instructions for UseBio-Beads S-X beads are supplied dry, and must beswollen prior to packing into a chromatographic column.The general instructions

4the Bio-Beads S-X beads will not swell, and the pore sizewill be minimal. The chosen solvent should be the one usedfor the separation, and the same a

5umn. If the amount of swelling is unknown, it can bechecked by swelling a known weight of beads and measur-ing the volume.After the beads are fully s

6Put together a clean column assembly. Place a smallamount of elution solvent in the column to prevent bubbleformation at the base of the poured colum

will be used during the separation. (This is not necessary forBio-Beads S-X1 beads.) Maintain at least a few centimeters ofliquid above the resin bed.