Bio-rad Bio-Plex Data Pro™ Software Manual do Utilizador

Bio-Plex Data Pro™Software User GuideVersion 1.0

Bio-Plex Data Pro Software User Guide | The Workspace6Data Menu CommandsThe following commands can be accessed from the Data menu:• Select Sample Type

The Workspace7View Menu CommandsFrom the View menu you can access the following:• Imported data sets in the Data Set Table• Individual sample data in

Bio-Plex Data Pro Software User Guide | The Workspace8

Bio-Plex Data Pro Software User Guide92 Working with ProjectsA project is the container for your data. After you create your project, you import data

Bio-Plex Data Pro Software User Guide | Creating a Project103. Type the name of the project owner in the Owner box.4. (Optional) Type the name of the

Opening a Project11The following information is displayed for each data set:Name — displays the name of the data set; by default this is the name of t

Bio-Plex Data Pro Software User Guide | Opening a Project122. In the Open Project dialog box, click on the project name.If the project contains data,

Editing a Project13Editing a ProjectYou can edit project attributes in the Edit project dialog box.To edit a project:1. Click Open Project.The Open Pr

Bio-Plex Data Pro Software User Guide | Organizing Your Projects14Organizing Your ProjectsOver the course of time, you will create multiple projects,

Bio-Plex Data Pro Software User Guide153 Working With Data SetsBio-Plex Manager™ software results files are imported into Bio-Plex Data Pro™ software

Bio-Plex Data Pro Software User Guide | iiBIO-RAD TECHNICAL SUPPORT DEPARTMENTThe Bio-Rad Technical Support Department in the U.S. is open Monday thro

Bio-Plex Data Pro Software User Guide | Importing Data Sets16Importing Data into Bio-Plex Data Pro SoftwareThe following procedure describes how to im

Importing Data Sets17Ext. Standards — the data were successfully imported. The unknown values were calculated using an external standard curve.Renamed

Bio-Plex Data Pro Software User Guide | Editing a Data Set18The Data File field displays the file name and indicates the source as Bio-Plex Manager. Y

Deleting a Data Set19A message asks you to confirm that you want to delete the data set.3. Click Yes. The data sets are deleted from the project.

Bio-Plex Data Pro Software User Guide | Deleting a Data Set20

Bio-Plex Data Pro Software User Guide214 Organizing the DataAfter data sets are imported into Bio-Plex Data Pro™ software, you can manage the data th

Bio-Plex Data Pro Software User Guide | Viewing and Editing Samples22Viewing and Editing SamplesThe Edit Samples/Add Columns view displays a tabular l

The Sample Table23The toolbar in the Edit Samples/Add Columns view displays additional icons.The toolbar also displays a drop-down list of all the dat

Bio-Plex Data Pro Software User Guide | About the Sample and Results Tables24About the Sample and Results TablesThe Sample and Results tables are sync

Filtering Data25To filter data:1. Click the triangle in the column header to open the drop-down menu.In the Filter section, there is a list of the dif

Bio-Plex Data Pro Software User GuideiiiTable of ContentsChapter 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1Ba

Bio-Plex Data Pro Software User Guide | Filtering Data26Filtering Data in the Perform Analysis/Create Charts ViewThe filter tool allows you to filter

Adding a Column27Removing FiltersTo clear filtering:• To clear filtering on a column, click the button in the column header to display the drop-down l

Bio-Plex Data Pro Software User Guide | Hiding a Column28To add a column to the Sample table:1. Click Add Column in the toolbar to open the drop-down

Sorting Data29To hide a column in the Results table:1. In the View Results/View Heat Map view, click Customize Result Table in the toolbar.2. In the C

Bio-Plex Data Pro Software User Guide | Sorting Data30Defining a Sort in the Sample or Results Tabl e sSorted data in the Sample or Results tables per

Sorting Data312. In the Sorting section, select Ascending or Descending to sort the data in the column.After a sort is applied to a column, an arrow a

Bio-Plex Data Pro Software User Guide | Sorting Data32Clearing Data SortingTo clear sorting on one column:• To clear sorting on the column, click the

Sorting Data33To clear sorting on all columns:• To clear sorting on all columns in the table, click Reset to Default Sort in the drop-down list for an

Bio-Plex Data Pro Software User Guide | Copying Data in Tables34Basic Sorting in Bio-Plex Data Pro SoftwareThis section describes how to sort Bio-Plex

Viewing Data Values with Full Precision35Copying the Data Set TableYou can copy the data set table to the clipboard and paste it into another applicat

Bio-Plex Data Pro Software User Guide | Table of ContentsivRemoving Filters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Bio-Plex Data Pro Software User Guide | Exporting Plots and Graphs36Exporting Plots and GraphsPlots and graphs can also be exported as Portable Networ

Bio-Plex Data Pro Software User Guide375 Viewing the DataThere are several different ways you can view your results in Bio-Plex Data Pro™ software. Y

Bio-Plex Data Pro Software User Guide | Viewing Results38Use this table to review the results of the experiment you imported. You can specify how you

Viewing Results39The Results table displays the following columns by default. You can add columns by clicking Add Column in the toolbar.Sample ID — yo

Bio-Plex Data Pro Software User Guide | Merging Data40Merging DataSamples may be run several times for one of the following reasons:• Dilution require

Merging Data41To me r ge res ult s dat a :1. In the Results table, choose “Merge samples run on different panels/assays” in the toolbar.2. In the Me

Bio-Plex Data Pro Software User Guide | Merging Data42Viewing Merged Data in the Results TableIn the Results table, the Merge column displays the numb

Merging Data43The multiple analyte view shows all the analytes and observed concentration merged values for each analyte.Viewing Merged Data in the Sa

Bio-Plex Data Pro Software User Guide | Heat Map Result View44Heat Map Result ViewA heat map is a graphical representation of data that enables you to

Heat Map Result View45Commands to the left of the heat map control the order of the samples and the labels used in the heat map. The Chart Options men

Bio-Plex Data Pro Software User Guide11 IntroductionBio-Plex Data Pro™ software provides tools for annotating and analyzing the results of experiment

Bio-Plex Data Pro Software User Guide | Heat Map Result View46HEAT MAP SETTINGSThe pane to the left of the heat map controls the labeling and sorting

Adding a Column47GROUPING DATA IN THE HEAT MAPYou can specify how data in the heat map are grouped. You can group your data by any column in the Resul

Bio-Plex Data Pro Software User Guide | Adding a Column48

Bio-Plex Data Pro Software User Guide496 Viewing the Results and Basic StatisticsIn the Perform Analysis/Create Charts view, you can view the graphs

Bio-Plex Data Pro Software User Guide | Analysis Workflow50Analysis WorkflowThe statistical analysis workflow comprises the following actions:• Choose

Analysis Workspace51• Analyte charts and tables — charts and tables that provide the results to the analysis you have configured using the controls in

Bio-Plex Data Pro Software User Guide | Interpreting P-Value Statistics52Analyte TableThe analyte table is the table at the top of this view. It conta

Interpreting P-Value Statistics53The smaller the P-value, the greater the probability that two or more groups of samples have significantly different

Bio-Plex Data Pro Software User Guide | Understanding Fold Change Statistics54Understanding Fold Change StatisticsFold change is calculated for each a

Analyte Charts and Tables55The following are additional options available on the toolbar:• Analytes — drop-down list of analytes in the data set.• Fil

Bio-Plex Data Pro Software User Guide | The Workspace2The WorkspaceThe Bio-Plex Data Pro software Home view displays buttons to create a new project o

Bio-Plex Data Pro Software User Guide | Analyte Charts and Tables56When the Auto checkbox is selected, the plot displays all groups inside the view po

Analyte Charts and Tables57When you select the Bar Graph icon, a Legend column appears in the analyte table. Each selected analyte corresponds to a co

Bio-Plex Data Pro Software User Guide | Analyte Charts and Tables58Summary TableThe Summary Table displays descriptive statistics for each sample grou

Analyte Charts and Tables59Export to Statistics SoftwareThis table displays each group as a column and includes only the sample values. Unlike the Res

Bio-Plex Data Pro Software User Guide | Analyte Charts and Tables60

Bio-Plex Data Pro Software User Guide61A Error MessagesAs you view your results in the Perform Analysis/Create Charts view using different measuremen

Bio-Plex Data Pro Software User Guide | 62OOR< OOR<Norm Ratio Acquisition Error No data present or not run Not Run No Bio-Plex Manager data for

Bio-Plex Data Pro Software User Guide63GlossaryAnalyteThe substance being measured, identified, quantitated, or otherwise analyzed in an experiment.

Bio-Plex Data Pro Software User Guide | 64P-valueStatistical representation of the chance that the relationship being observed is observed by pure cha

Bio-Plex Data Pro Software User Guide65IndexSymbols%CV 39AActive projectdefinition of 14Addingcolumns 27–28Analysis Configuration pane 50, 51Analytede

The Workspace3Overflow Toolbar CommandsClicking the triangle displays an overflow toolbar of hidden commands. Overflow toolbars are enabled whenever t

Bio-Plex Data Pro Software User Guide | 66importing, from Bio-Plex Manager17–18importing, status of 16merging 40–43row, copying 34rows, combining 40so

67Mergingdata 40–43Muilti Analyte tablefiltering data in 24Multi Analyte table 39OObs Conc 38Obs Conc column 39Observed Concentration 42One-way/ANOVA

Bio-Plex Data Pro Software User Guide | 68Summary table 54TToolbarAnalyte table 52Bio-Plex Data Pro 2Edit Samples/Add Columns view23Overflow 3t-test 5

Life ScienceGroup11-1373 0911 Sig 021110022871 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

Bio-Plex Data Pro Software User Guide | The Workspace4File Menu CommandsThe following commands can be accessed from the File menu:• New Project — open

The Workspace5• Customize Sample Table — opens a dialog box in which you can select or clear columns or add new columns to the sample table.• Customiz