Bio-rad Biofuel Enzyme Kit Manual do Utilizador

Quick GuideActivity 1: Determine the ReactionRate in the Presence or Absenceof an Enzyme1. Find your 15 ml conical tubes labeled“Stop Solution”, “1.5

Quick GuideActivity 6: Test the Ability ofMushroom Extracts to Increasethe Reaction Rate1. Write down the name of your mushroom______________________

7. Using a clean DPTP, pipet 500 µl of stopsolution into each cuvette. Rinse out theDPTP thoroughly with water.8. Label a 15 ml conical tube with th

Life Science Group 10-0100 0210 Sig 11095947 Rev A US/EGBio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61

Read and understand steps 8–11 fullybefore proceeding. These steps are timesensitive!8. Using the DPTP labeled “C”, pipet 500 µlof buffer into the 15

Quick GuideActivity 2: Determine the Effect ofTemperature on the Reaction Rate1. Label your cuvettes “0°C”, “22°C”, and“37°C”. 2. Using a clean DPTP

6. Have a stopwatch ready. Using a cleanDPTP, pipet the 250 µl of enzyme fromthe tube labeled “0°C Enzyme” into thetube labeled “0°C Substrate”, and

Quick GuideActivity 3: Determine the effect ofpH on the Reaction Rate1. Label your cuvettes “pH 5.0”, “pH 6.3”,and “pH 8.6”. 2. Using a clean DPTP,

Quick GuideActivity 4: Determine the Effect ofEnzyme Concentration on theReaction Rate1. Label one 15 ml conical tube “LowConcentration Enzyme”. Usin

Please read steps 5–7 fully before proceeding. These steps are time sensitive!5. Using the DPTP labeled with an “H”,pipet 250 µl of 1.5 mM substrate

Quick GuideActivity 5: Determine the Effect ofSubstrate Concentration on theReaction Rate1. Label one clean 15 ml conical tube “LowConcentration Subs

Please read and understand steps 7–9fully before proceeding. These steps aretime sensitive!7. Using a clean DPTP, pipet 750 µl ofenzyme into your 15