Bio-rad Bio-Rex® Reactor Grade Resins Manual do Utilizador

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules CA 94547LIT205 Rev BAG®501-X8 and Bio-Rex®MSZ 501(D)Mixed Bed ResinInstruction ManualLIT205B 6

1IntroductionMixed bed resins are used for deionizing water orother non-ionic substances such as urea, acrylamide, for-mamide, or glyoxal. Deionizatio

Instructions for UseThe batch method or the column method may be usedto deionize a sample with mixed bed resin.Batch MethodThe batch method is the add

56. Slowly pour the solution to be deionized into thereservoir above the column, then elute slowly throughthe column taking care not to disturb the re

Regenerate the cation exchanger using 3 bed volumesof 3 N HCl and rinse with 4 bed volumes of deionizedwater or until the effluent is >pH 5. Regene

Note: Formamide interferes with the color change ofthe dye, but will not affect the deionization capacityof the resin.Batch vs. Column Deionization of

Fig. 2. Separation of hemoglobin from ampholytesand sucrose on a mixed bed ion exchange column(AG 501-X8 resin). A hemoglobin blank in the sucrosedete

References1. Kapp, O. H. and Vinogradov, S. N., Anal. Biochem., 91, 230 (1978).2. Bakker, J. A., Vanden Brande, J. L. and Haggerbrugge, C. M., J. Ch