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For Calibration of

Hydrophobic Columns

The Protein Standard for Hydrophobic Chroma-

tography is a lyophilized mixture of proteins which

exhibit different hydrophobic behavior. It may be used as

a calibration standard for HIC columns for analysis under

nondenaturing conditions, and is appropriate for

calibrating some reverse phase columns as well. The

mixture contains cytochrome c, myoglobin and

lysozyme. The standard is supplied as a set of 6 vials of

lyophilized protein mixture. The total protein content is

approximately 3 mg per vial.

Instructions

Rehydrate the standard by adding 0.5 ml of starting

buffer to the vial. Swirl gently to mix and allow the vial

to stand for 2–3 minutes. Swirl the vial again; centrifuge

or filter to remove fine particulates before injection.

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Bio-rad Chromatography Standards Manual do Utilizador Página 2