Bio-rad Quantum Prep Plasmid Kits Manual do Utilizador descarregar pdf

Quantum Prep

™

Plasmid Midiprep Kit

Instruction Manual

Catalog Number

732-6120

For Technical Service

Call Your Local Bio-Rad Office or

in the U.S. Call 1-800-4BIORAD

(1-800-424-6723)

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547

4100111 Rev E

4100111E 5/3/99 8:25 AM Page Cvr2

1 2 3 4 5 6

Resumo do Conteúdo

Página 1 - Instruction Manual

Quantum Prep™Plasmid Midiprep KitInstruction ManualCatalog Number732-6120For Technical ServiceCall Your Local Bio-Rad Office orin the U.S. Call 1-800-

Página 2 - Table of Contents

Table of ContentsSection 1 Introduction ...11.1 Overview...

Página 3 - Introduction

Section 1Introduction1.1 OverviewThe Quantum Prep plasmid midiprep kit has beenoptimized for the rapid purification of DNA from 40 to 50 ml of liquid

Página 4 - Protocol

plasmids, as that will not usually result in higher yields usingthis protocol. For low-copy number plasmids (e.g. pBR322),up to 60 ml of culture can b

Página 5 - 2.2 Protocol

5. Add 5 ml of Neutralization Solution. Cap the tube and mixby inverting the tube 6–8 times. The solution shouldbecome cloudy and develop a flocculant

Página 6 - Product Information

Section 3References1. U.S. Patent 5,075,430 issued to Bio-Rad Laboratories.2. Ausubel et al., Current Protocols in Molecular Biology,Wiley-Interscienc