Bio-rad Fish DNA Barcoding Kit Manual do Utilizador

Biotechnology Explorer™Fish DNA Barcoding KitQuick Guideexplorer.bio-rad.comCatalog #166-5100EDUDuplication of any part of this document is permitted

8 Fish DNA Barcoding Quick GuideQuick GuideLesson 4: Sequencing 1. Parafilm your capped Fish 1 SEQ, Fish 2 SEQ, and (+) SEQ tubes thoroughly to pre

Life ScienceGroup Sig 1212Bulletin 6410 Rev A US/EG 13-1028 0513Bio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

Fish DNA Barcoding Quick Guide 1 Quick GuideLesson 1: DNA ExtractionPreparing Fish Samples1. Label one capped 2 ml microcentrifuge tube for each o

2 Fish DNA Barcoding Quick GuideExtracting DNA from fish samples1. Add 200 µl of Resuspension to your two microcentrifuge tubes containing minced fi

Fish DNA Barcoding Quick Guide 3 8. Transfer the entire supernatant (500–550 µl)of each fish sample from step 5 into the appropriately labeled spin

4 Fish DNA Barcoding Quick Guide15. Remove the spin column from the 2 ml microcentrifuge tube, discard the flowthrough at the bottom of the 2 ml tu

Fish DNA Barcoding Quick Guide 5 Quick GuideLesson 2: PCR Amplification of DNA1. Label four PCR tubes with your initials and the sample name (1 for

6 Fish DNA Barcoding Quick GuidePreparing PCR Samples for Electrophoresis and Sequencing1. Label four 2 ml microcentrifuge tubes with both your in

Fish DNA Barcoding Quick Guide 7 Quick GuideLesson 3: Gel Electrophoresis1. Retrieve the 5 µl samples of PCR products (4 samples) from 4°C. To eac