Bio-rad RC DC™ Protein Assay Manual do Utilizador descarregar pdf (Página 4)

3 Pipet 25 µl of standards and samples into clean, dry microfuge tubes.

4 Add 125 µl RC Reagent I into each tube, vortex. Incubate the tubes for 1

minute at room temperature.

5 Add 125 µl RC Reagent II into each tube, vortex. Centrifuge the tubes at

15,000xg for 3-5 minutes.

6 Discard the supernatant by inverting the tubes on clean, absorbent tissue

paper. Allow the liquid to drain completely from the tubes.

7 Add 127 µl Reagent A´ to each microfuge tube, vortex. Incubate tubes at room

temperature for 5 minutes, or until precipitate is completely dissolved. Vortex

before proceeding to the next step.

8 Add 1 ml of DC Reagent B to each tube and vortex immediately. Incubate at

room temperature for 15 minutes.

9 After the 15 minutes incubation, absorbances can be read at 750 nm. The

absorbances will be stable for at least 1 hour.

Section 5 Storage

RC Reagent I and RC Reagent II should be stored at room temperature (20°C to

30°C) away from direct sunlight. Both reagents have a shelf life of 12 months.

(Also see DCProtein Assay Instruction Manual for storage conditions for DC

Protein Assay Reagent A, Reagent B and Reagent S.)

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Bio-rad RC DC™ Protein Assay Manual do Utilizador Página 4