Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Manual do Utilizador descarregar pdf (Página 23)

18. Pipet 40 µl (or 30 µl)

†

of the elution solution onto the center of

the membrane at the bottom of the RNA binding column.

†

Note: When isolating total RNA from small amounts of starting material

(<10 mg of tissue or 500,000 cells), perform a single elution with 30 µl

of the elution solution. Do not perform step 21.

19.Incubate 1 min for complete soaking and saturation of the

membrane.

20. Centrifuge for 2 min at >12,000 x g to elute the total RNA.

21. Repeat steps 18 and 19 using 40 µl of the elution solution if

the starting amounts of starting material is more than 10 mg of

tissue or 500,000 cells.

Note: The eluted total RNA samples can be used immediately in downstream

applications. Alternatively, the RNA sample can be aliquoted and stored at

–20°C or –70°C for later use.

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Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Manual do Utilizador Página 23