Bio-rad Aurum™ Total RNA Mini Kit Manual do Utilizador descarregar pdf (Página 25)

Problem Possible Cause Recommended Solution

Total RNA prep Incorrect use of wash Add the appropriate

performs poorly solutions volume of 95–100%

in downstream ethanol to the wash

applications solutions before initial

use

RNA is degraded See problem “RNA

degradation”

Ethanol contamination Add 1–3 min to the

in prep (eluate volumes centrifugation time

>80 µl) after the final wash step

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Bio-rad Aurum™ Total RNA Mini Kit Manual do Utilizador Página 25