Bio-rad Prepacked Gravity Flow Columns for Ion Exchange Manual do Utilizador

Prefilled Poly-Prep®Columns for Ion ExchangeChromatographyInstruction ManualLIT84B 9/1/98 8:36 AM Page FC1

Section 9Ordering InformationCatalog Ionic MeshNumber Column Packing Form Size731-6211 AG 1-X8 Resin, 50 Cl–100-200731-6212 AG 1-X8 Resin, 50 Cl–200-4

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT84 Rev BLIT84B 9/1/98 8:37 AM Page BC1

Table of ContentsSection 1 Introduction ...1Section 2 Column Description...2Section 3 Instructions For Use...

Section 1IntroductionPrefilled Poly-Prep columns are smallpolypropylene columns containing ionexchange resins. These columns combine theanalytical qua

Poly-Prep columns are ideal for routineapplications in which a small amount of chro-matographic medium is used and then dis-carded, or for multiple sa

2. Remove the column cap to allow the eluantto flow freely.3. Snap off the seal at the bottom of thecolumn.4. Monitor the level of liquid in the reser

The ion exchange capacity, usuallyexpressed as meq/ml of resin, is equivalent tothe number of ion exchange sites per unit vol-ume of resin. The follow

Section 6Chemical CompatibilityTable 2 gives the chemical compatibilityof prefilled Poly-Prep columns. While the col-umn is often very resistant, the

Section 7Temperature LimitsIncreases in temperature tend to decreaseresin selectivity, and can be used to decreaseelution times. Increased temperature

can be removed easily by rinsing with 2-3bed volumes of mobile phase.3. Effluent is red: After prolonged storage,sulfonic acid will sometimes leach fr