Bio-rad Nuvia™ IMAC Resin Manual do Utilizador

Bio-Scale™ Mini Nuvia™ IMAC Ni-ChargedCartridges, 1 and 5 mlInstruction ManualCatalog numbers780-0811780-0812

6 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 4Connection to Medium- and High-Pressure Chromatography SystemsBio-Scale Mini Cartridges can be conne

Bio-Scale Mini Nuvia IMAC Ni-Charged 7 Section 5Buffers and MethodsIMAC methods can be run using either native or denaturing purification protocols.

8 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 6Quick Solubility Screening ProtocolsBefore choosing a native or denaturing purification protocol, it

Bio-Scale Mini Nuvia IMAC Ni-Charged 9 Section 7Preparation of E. coli LysatesFor E. coli cultures expressing medium to high levels of histidine-ta

10 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 8Preparing a Cartridge and Subsequent PurificationPrepare buffer sets for either the native or denatu

Bio-Scale Mini Nuvia IMAC Ni-Charged 11 2. Load the sample lysate at 1 ml/min.3. Wash the cartridge with 6 CV of wash buffer 1 at 1 ml/min.4. Wa

12 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 9Scaling UpBio-Scale Mini Cartridges are available in 1 and 5 ml cartridge formats. The Nuvia IMAC R

Bio-Scale Mini Nuvia IMAC Ni-Charged 13 Section 10Regenerating, Cleaning, Sanitizing, and StoringProtein cross-contamination, frit clogging, and in

14 Bio-Scale Mini Nuvia IMAC Ni-ChargedIn situations where it is desirable to run different proteins over the same cartridge, completely sanitize,

Bio-Scale Mini Nuvia IMAC Ni-Charged 15 Section 11Troubleshooting Guide Cartridge clogging or slow flow rateParticulates in samples Filter all sampl

16 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 12Ordering InformationBio-Scale Mini Nuvia IMAC Ni-Charged CartridgesCatalog # Description780-0811 B

Bio-Scale Mini Nuvia IMAC Ni-Charged 17 Section 13ReferencesJoyce AR and Palsson BO (2006). The model organism as a system: integrating ‘omics’ dat

18 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 14Legal NoticesTriton is a trademark of Union Carbide. Tween is a trademark of ICI Americas, Inc.

Life ScienceGroupSig 121310041106 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austr

Table of ContentsSection 1 Introduction... 1Section 2 Product Information...

Bio-Scale Mini Nuvia IMAC Ni-Charged 1 Section 1IntroductionBio-Scale™ Mini Nuvia™ IMAC Ni-Charged Cartridges are convenient, disposable, prepacked

2 Bio-Scale Mini Nuvia IMAC Ni-ChargedSection 2Product InformationBio-Scale Mini Cartridges are disposable, easy-to-use, prepacked chromatographic

Bio-Scale Mini Nuvia IMAC Ni-Charged 3 Table 2. Nuvia IMAC Ni-Charged Resin specifications. Functional ligand Nitrilotriacetic acid (NTA)Base bead U

4 Bio-Scale Mini Nuvia IMAC Ni-ChargedNuvia IMAC Cartridges are compatible with the aqueous buffers most commonly used with IMAC purification techni

Bio-Scale Mini Nuvia IMAC Ni-Charged 5 Section 3Connection to Low-Pressure Chromatography SystemsBio-Scale Mini Cartridges are ideal for use with a