Bio-rad Nuvia™ IMAC Resin Manual do Utilizador descarregar pdf (Página 18)

14 Bio-Scale Mini Nuvia IMAC Ni-Charged

In situations where it is desirable to run different proteins over

the same cartridge, completely sanitize, strip, and recharge the

cartridge between sample runs. Take care when handling and

disposing of metal-containing solutions.

1. Clean the cartridge with 10 CV of 1 M NaOH.

2. Rinse the cartridge with 10 CV water.

3. Strip metal ions with 5 CV of 0.1 M EDTA.

4. Rinse the cartridge with 10 CV water.

5. Recharge the cartridge with 5 CV of 0.1 M nickel sulfate

pH 4.5.

6. Rinse the cartridge with 10 CV water.

7. Store the cartridge in 20% ethanol.

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Bio-rad Nuvia™ IMAC Resin Manual do Utilizador Página 18