Bio-rad Nuvia™ IMAC Resin Manual do Utilizador descarregar pdf (Página 8)

4 Bio-Scale Mini Nuvia IMAC Ni-Charged

Nuvia IMAC Cartridges are compatible with the aqueous buffers

most commonly used with IMAC puriﬁcation techniques.

Table 3. Chemical compatibilities for Nuvia IMAC Ni-Charged

Cartridges.*

Reagent Stability

Chelating agent

EDTA 1 mM

Sulfhydryl reagents

β-mercaptoethanol 20 mM

DTT 10 mM

TCEP 20 mM

Detergents

Nonionic

(Triton, Tween)

Zwitterionic

(CHAPS, CHAPSO)

Denaturing agents

Guanidine-HCl 6 M

Urea 8 M

Other additives

NaCl 2 M (include at least 300 mM NaCl in buffers)

MgCl

100 mM (use HEPES to prevent precipitation)

CaCl

5 mM (use HEPES to prevent precipitation)

Glycerol 20% (backpressure may increase signiﬁcantly,

slower ﬂow rates may be required)

Ethanol 20%

* Nuvia IMAC binding capacities are unaffected up to the concentrations given when employing

typical reagents used for histidine-tagged protein puriﬁcation.

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