Bio-rad Nuvia™ IMAC Resin Manual do Utilizador descarregar pdf (Página 7)

Bio-Scale Mini Nuvia IMAC Ni-Charged 3

Table 2. Nuvia IMAC Ni-Charged Resin speciﬁcations.

Functional ligand Nitrilotriacetic acid (NTA)

Base bead UNOsphere

™

Particle size range 38–53 μm

Mean particle size 50 μm

Metal ion capacity ≥18 μmol Cu

/ml

Dynamic binding

capacity*

≥40 mg/ml

Recommended ﬂow

rate

480 cm/hr

Maximum operating

pressure

45 psi

Chemical compatibility See Table 3

Storage 4°C to ambient temperature

Storage

recommendations

20% ethanol

Shelf life in 20% ethanol >3 years at ambient temperature

Operational

temperature range

4–40°C

Autoclavability Not autoclavable

* Q10% determination of 1 mg/ml (His)6-tagged pure protein (40 kD). Note: Dynamic binding

capacity will vary from protein to protein.

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