Bio-rad ProteinChip Matrices Manual do Utilizador descarregar pdf (Página 4)

Table 1. Preparation of ProteinChip EAM in solution using

commonly used solvent systems.

EAM Solvent System Preparation

ProteinChip CHCA 50% acetonitrile

49.75% HPLC

grade H

0.25% trifluoroacetic

acid (TFA)

ProteinChip SPA 50% acetonitrile

49.50% HPLC

grade H

0.50% TFA

ProteinChip EAM-1 50% acetonitrile

49.50% HPLC

grade H

0.50% TFA

Preparation

1. Add 200 µl solvent to vial

(there should be undissolved

CHCA in the bottom of the

tube)

2. Mix

3. Centrifuge

4. Draw off supernatant and

dilute with equal part solvent

1. Add 400 µl solvent to vial

2. Mix

3. Centrifuge

1. Add 200 µl solvent to vial

(there should be undissolved

EAM-1 in the bottom of the

tube)

2. Mix

3. Centrifuge

4. Draw off supernatant and

dilute with 2x volume of

solvent

1 2 3 4 5 6 7 8

Sem comentários

Bio-rad ProteinChip Matrices Manual do Utilizador Página 4