Bio-rad UNO® Monolith Cation Exchange Columns Manual do Utilizador

For Technical Service Call Your Local Bio-Rad Officeor in the U.S. Call 1-800-4BIORAD (1-800-424-6723)UNO™ Q&S PolishingColumn for Continuous BedI

Bio-Rad LaboratoriesU.S. (800) 4BIORAD California (510) 741-1000 Australia 02-9914-2800 Austria (1) 877 89 01 Belgium 09-385 55 11 Canada (905) 712-2

UNO Q & S Polishing Ion Exchange ColumnIntroductionUNO prepacked ion-exchange columns are designed to meet the needs of thebio-chromatographer for

2use of inert, biocompatible (ceramic, PEEK, titanium) solvent delivery systemsfor maximum column life and recovery of sample biological activity.Tabl

while maintaining maximum buffering capacity. In any case, a buffer concentrationof 20 mM is recommended.As can be seen in Table 2 and 3, the pKaand h

Sample Preparation Proper adjustment of the sample pH and ionic strength is critical for consistentand reproducible chromatography. For best results,

Gradient Volumes & Salt ConcentrationsAs a starting point for developing a separation, we recommend using the UNOQ Polishing column with a simple

concentration (CMC). As the salt concentration (i.e. the counter-ion concentration)increases, the CMC drops and eventually micelles will form. This ma

Storage ConditionsPrior to long-term storage, the column should be cleaned as previously describedand then washed with 5 ml of 20% ethanol. This will

CatalogNumber Product Description751-0091 Bio-Scale 2 Replacement Part Kit, includes 5 frits, 5 distri-bution screens, 2 o-rings, 1 frit remover. Use