Bio-rad Aurum™ Total RNA 96 Kit Manual do Utilizador descarregar pdf (Página 20)

Problem Possible Cause Recommended Solution

Low or highly variable Elution solution applied Apply elution solution

eluate volumes among to RNA binding plate well directly to membranes at

wells walls base of each well

RNA binding plate not Set plate properly and

seated properly press down gently to

seat

Abrupt application of Increase negative

negative pressure during pressure gradually

elution over 5–10 sec using

the vacuum regulator

   Residualwashbufferon BlotRNAbindingplate

drip directors drip directors with paper

towels; ensure that the

plate underside is dry

Genomic DNA Incomplete DNase I Increase DNase I digestion

contamination digestion time

Inactive DNase I Store reconstituted

DNase I in a nonfrost-

free freezer; avoid

freeze-thaw cycles;

aliquot reconstituted

DNase I for single use

only

Excessive amount of Reduce volume of

starting material per well culture used

Incorrect preparation Use only the DNase

of DNase dilution dilution solution provided

in the kit to dilute the

DNase

RNA degradation RNase contamination of DEPC-treat all handmade

user-made solutions solutions; decontaminate

  and/orplasticware allworksurfaces;see

Section 5 for more

details

Endogenous RNases Work quickly through

the steps prior to the

addition of lysis solution

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Bio-rad Aurum™ Total RNA 96 Kit Manual do Utilizador Página 20