Bio-rad Aurum™ Total RNA 96 Kit Manual do Utilizador descarregar pdf (Página 3)

Table of Contents

Section 1 Introduction.............................1

Section 2 Kit Components .........................1

Section 3 Storage Conditions .......................2

Section 4 Necessary Supplies.......................2

Section 5 Before Using the

Aurum™ Total RNA 96 Kit...................3

Starting Material Amounts .......................3

Reagents Used With the Aurum™ Total RNA 96 Kit ...4

Elution Guidelines ..............................5

Ribonucleases ................................5

Disruption and Homogenization ...................6

Section 6 Vacuum Manifold Setup and Use With

96-Well Plates...........................6

Guidelines for Vacuum Format ....................6

Preparing the Aurum™ Vacuum Manifold............7

Vacuum Setup ................................7

Manifold Wash Setup ...........................7

Manifold Elution Setup ..........................8

Section 7 Vacuum Protocol.........................9

Section 8 Spin Protocol ..........................12

Section 9 Troubleshooting Guide ...................15

Section 10 Ordering Information.....................19

1 2 3 4 5 6 7 8 ... 23 24

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Bio-rad Aurum™ Total RNA 96 Kit Manual do Utilizador Página 3