Bio-rad Chromatographic Surfaces Manual do Utilizador descarregar pdf (Página 4)

Recommended Binding and Washing Solutions

ProteinChip H50 buffer (catalog #K20-00001)

(10% acetonitrile, 0.1% trifluoroacetic acid (TFA))

0–50% methanol or acetonitrile ± 0.1–1% TFA

0.1% TFA may be added to the binding solution to

increase binding

Fractionation for Serum Profiling

Fractionation of serum prior to profiling is recommended. The

fractionation procedure produces 6 fractions containing proteins

separated on the basis of their isoelectric point. Fractionation

allows segregation of highly abundant proteins into a limited

number of fractions, thereby reducing signal suppression effects

on lower-abundance proteins.

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