Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 171)

Creating a Method from Standard Phases

User Guide | 169

Serpentine is selected by default. Fractions can also be collected by rows or

columns for microplates. Possible choices appear for the rack you select.

If you have outlet valves in the fluidic scheme, choose a starting valve and port

and modify the fraction size.

8. Under Flow, specify the default flow rate for the method. You can change the

default flow rate within individual phases if needed.

Select the Control the flow to avoid overpressure checkbox if you want the flow

rate to decr

ease and the method to continue running in the event that the

system r

eaches high pressure limits. If you do not select this checkbox the

system pumps will stop, pausing the method.

If you have an air sensor installed and you want to monitor and stop the system

pumps if air is detected in the lines, select the Detect end of buf

fer with Air

Sensor checkbox.

Note: If the method pauses due to an end-of-buf

fer signal, the fluidic lines

must be primed befor

e r

esuming the method. Because the system pump’s

Purge button is inactive in Method mode, you must reprime the pumps

manually. See Priming and Purging the System on page 94 for information

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Bio-rad ChromLab™ Software Manual do Utilizador Página 171