Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 231)

Peak Integration

User Guide | 229

Peak Parameters

You can specify initial values for two parameters that participate in the peak

detection process: slope and filter sensitivity.

Slope — slope defines the thr

eshold for peak slope. The bigger the slope, the

steeper the peaks that are filtered out. Slope values range from 1–100.

Sensitivity — sensitivity determines the str

ength of the filter used for smoothing the

curve before finding peaks. The Low sensitivity setting filters out higher frequency

components in the signal, eliminating small, narrow peaks. The higher the sensitivity

setting, the more higher frequency components get through the filter. This allows

smaller and narrower peaks to be detected.

Peak Filtering

You can specify the following thresholds for valid peaks.

Min Height — this setting specifies the minimum height for a valid peak in mAU

units.

Min Width — this setting specifies the minimum baseline width for a valid peak in

ml units.

N-Largest — this setting specifies ar

ea-wise filtering. It picks N largest peaks in

descending order.

Range — these settings define the range within which peaks will be detected. The

range units ar

e determined from the chromatogram x-axis units.

Default Parameters

Reset — restores the baseline parameters, peak detection parameters, and peak

filtering setting to their defaults.

Note: You must click Integrate to recalculate peaks with the default settings.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 231