Bio-rad Econo Gradient Pump Kits Manual do Utilizador

Consulte online ou descarregue Manual do Utilizador para Instrumentos de medida Bio-rad Econo Gradient Pump Kits. Bio-Rad Econo Gradient Pump Kits User Manual Manual do Utilizador

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Página 1 - Instruction Manual

ECONOGRADIENTPUMPEcono Gradient PumpInstruction Manual4006161B.qxd 8/14/2003 8:09 AM Page 1

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Front Panel FunctionCancel KeyPressing Cancel will clear an entry that hasbeen input with either the arrow or soft keys.If no entry has been made, the

Página 3 - TABLE OF CONTENTS

Front Panel FunctionPurge KeyThis key is used to prime and purge tubinglines without disturbing the primary speedsetting of the pump. When Purge is pr

Página 4 - Table of Contents

3.2 REAR PANEL FUNCTIONSFigure 3-2. Rear panel connectors.Display FunctionPower Entry ModuleGrounded 3-pin receptacle for the powercord. Also containe

Página 5 - 1.0 INTRODUCTION

Display FunctionAux Valve ConnectorThis 8-pin connector controls auxiliaryvalves such as the optional SV-3 Divertervalve or the optional Splitter valv

Página 6 - 2.0 UNPACKING AND SETTING UP

4.0 TUBING SELECTION AND INSTALLATION4.1 TUBING SELECTIONThe Econo Gradient Pump may be used with most flexible tubinghaving an inner diameter less t

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When using tubing other than precut tubing from Bio-Rad, the tubingmust be cut to the following lengths to accommodate prestretch:Tubing LengthTygon,

Página 8 - FEATURES

4.2 TUBING INSTALLATION1. Referring to Figure 4-2, pull the platen cam lever away from thepump head to unlock the platen and slide the platen away fr

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3. Insert one end of the tubing into the tubing retaining bracket ofthe pump head. Lightly pull the tubing around the rollers toremove slack. Attach t

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5.0 SYSTEM OPERATIONThere are four modes of system operation:• Remote• Setup• Manual• Program, including running the system5.1 REMOTE MODEThe Econo

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Screen 1. Flow Rate Selection.Use the soft keys to select either RPM (revolutions per minute) orml/m. This information will be used in writing a prot

Página 12 - Made in U.S.A

SAFETYCaution/WarningDisconnect power to the Econo Gradient Pump beforeservicing. No user-serviceable parts are inside the unit.Refer servicing to Bi

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System Operation16Screen 4. Pump Calibration by Measured Flow.Pressing the left or right soft keys will start the pump for the selectedtime. Collect

Página 14 - FLOW RATE RANGE (ml/min)

Screen 6. Selecting Units for Programming.Programming steps can be described in either units of time (inminutes) or volume (in ml).6 Program Steps TI

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Screen 9. Biologic System Address Number.The Econo Gradient Pump can be connected to the BioLogic HR orBioLogic Duo-Flow workstation via an Instrumen

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Screen 12. Beeper.The beeper can be switched on or off at the user’s discretion. Thebeeper, which beeps on each key press, can be disabled.12 Beep

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5.3 MANUAL MODEThe Econo Gradient Pump and attached devices can be controlledusing the pump’s front panel membrane keys and LCD display whilein Manual

Página 18 - 5.0 SYSTEM OPERATION

5.3.3 Setting Auxiliary (SV-3 or Splitter) Valve ControlControl for an auxiliary valve is selected by pressing the right soft key.This option is avail

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Each field may be selected with the corresponding soft key. Thearrow keys are then used to increment or decrement the value.Pressing the Step key or t

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5.5 RUNNING A PROGRAMTo start a program (be sure to enter the Program mode by using theMode key), press the Run key. This will start the run. By usin

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5.5.1 Holding a RunPressing the Hold key displays the following screen:Holding ___%B CONTINUE Abort When holding, the pump runs indefinitely at the

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5.5.4 Power Failure While in Run ModeIn the event of a power failure while a Program is running, the LCDdisplay will indicate:Power Failure! CONTINUE

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TABLE OF CONTENTSSafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iSection 1 Introduction . . . . . . . . . . . . . . . . . . . .

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6.0 OPERATION WITH OTHER BIO-RADINSTRUMENTSThe Econo Gradient Pump (EGP) may be used with otherchromatography components (the Model 2110 or Model 212

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Model 2128 Fraction Collector (continued)Alternatively, one may use the Model 2128 DiverterValve; it will respond to the program in the Model2128 Frac

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Model 2110 Fraction Collector (continued)When a programmed run is started, the EGP willautomatically start the fraction collector. If the SV-3diverter

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Model 1327 Chart Recorder (continued)If PAUSE is selected during the program, then thepump and chart recorder will stop.The pump and chart recorder w

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BioLogic Duo-Flow SystemThe EGP can be controlled by the BioLogic Duo-FlowSystem provided that the BioLogic System firmwareis version 3.0 or higher.Co

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Figure 6-1. System Cabling.Other Instruments31ECONOGRADIENTPUMPMODEL 2110 FRACTION COLLECTORORMODEL 2128 FRACTION COLLECTORMODEL 1327CHART RECORDERCAB

Página 30 - INSTRUMENTS

Figure 6-2. System Plumbing.Other Instruments32ECONOGRADIENT PUMPMODEL 2110FRACTIONCOLLECTORMODEL 2128FRACTIONCOLLECTORMODEL EG-1GRADIENTMONITORECONO

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7.0 CLEANING AND MAINTENANCEThe Econo Gradient Pump requires very little maintenance to assurereliable operation. The procedures outlined below will

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4. The fuse socket in the fuse drawer is removable. Turn the socketsuch that the correct voltage shows through the window.5. Reinsert the fuse drawer

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8.0 TROUBLESHOOTINGProblem Possible Cause SolutionPump displays Power outage If you wish to continue a run in“Power Failure!” progress, press the Co

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Appendix C Warranty . . . . . . . . . . . . . . . . . . . . . . . 43Appendix D Ordering Information . . . . . . . . . . . . . 44Table of Contentsiv4

Página 35 - CABLE 22 OR 23

Problem Possible Cause SolutionFlow rate not Pump tubing Replace tubing.consistent damaged or worn outPlaten adjustment Adjust the platen by turningin

Página 36 - ECONO GRADIENT PUMP

Problem Possible Cause SolutionCannot Pump must be Insure that pump is stopped.calibrate stopped in order topump calibrate pumpRPM has been Select

Página 37 - 7.0 CLEANING AND MAINTENANCE

9.0 EMBEDDED FUNCTIONSDuring a technical service call, you may be asked to perform taskswhich require you to press combinations of keys. These tasks

Página 38 - Fuse Drawer

APPENDIX ATECHNICAL SPECIFICATIONSNumber of channels 2Flow rate range (per channel) 0.002 - 20 ml/min (depending ontubing diameter)Pump head speed 0.0

Página 39 - 8.0 TROUBLESHOOTING

Gradient Mixer Range 0-100% BIncrement 1% BValve controlSV-3 Diverter Valve Waste and divert positions.Splitter Valve 0-100%Fraction CollectorsModel 2

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APPENDIX BCONNECTION TO OTHER INSTRUMENTSThe I/O connector on the rear panel of the pump is for the operationof the fraction collectors and chart reco

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Selected Fraction CollectorPin# Name None 2110 21285 no contact no contact no contact6 ER/PD held LOW held LOW held LOWstarts 1327 starts 1327 starts

Página 42 - 9.0 EMBEDDED FUNCTIONS

APPENDIX C WARRANTYThe Econo Gradient Pump is warranted for one year against defectsin materials and workmanship. If any defects should occur during t

Página 43 - TECHNICAL SPECIFICATIONS

APPENDIX DORDERING INFORMATIONCatalogNumber Product DescriptionEcono Gradient Pump731-9001 Econo Gradient Pump 100/120 V731-9002 Econo Gradient Pump 2

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CatalogNumber Product DescriptionTubing and Accessories731-8210 Silicone Tubing, 0.8 mm ID, 0.8 mm wall, 10 m731-8211 Silicone Tubing, 1.6 mm ID, 0.8

Página 45 - APPENDIX B

1.0 INTRODUCTIONThe Econo Gradient Pump (EGP) is a two-channel, bi-directional,variable speed peristaltic pump for low-pressure chromatography andgen

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CatalogNumber Product Description731-9006 Low Pressure, Small Fittings Kit. Contains a subset ofthe fittings found in catalog # 731-8220, including ba

Página 47 - WARRANTY

Life ScienceGroupWeb sitewww.bio-rad.com USA(800) 4BIORAD Australia 02 9914 2800 Austria(01) 877 89 01 Canada(905) 712-2771 45 44 52-1000 Finland089

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2.0 UNPACKING AND SETTING UP2.1 UNPACKING INSTRUCTIONSCarefully remove the unit from the shipping box, lifting from thehandle on the back of the pump

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Your Econo Gradient Pump was carefully tested at the factory andwas shipped in good working order. If any part is missing ordamaged, contact your loca

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3.0 PHYSICAL DESCRIPTION AND CONTROLFEATURES3.1 FRONT PANEL FUNCTIONSFigure 3-1. Front Panel Controls and Displays.Description4ECONO GRADIENT PUMPRe

Página 51 - 4006161 Rev B

Front Panel Function[LCD; see figure 3-1] LCD DisplayThis display has two rows of 16 characters.Different windows will appear on this displaydepending

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