Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manual do Utilizador

ENrich™ QENrich™ SHigh-Resolution Ion Exchange ColumnsInstruction ManualCatalog numbers780-0001780-0003780-0021780-0023 Please read these instructions

6 ENrich Instruction Manual Gradient Volumes and Salt ConcentrationsAs a starting point for developing a separation, we recommend using the ENrich c

ENrich Instruction Manual 7Section 3: Care of the ENrich Ion Exchange Column 3.1 Column Cleaning Careful preparation (especially filtration) of th

8 ENrich Instruction Manual 3.2 Bed Height Adjustment Under certain conditions of buffer composition, high flow rates or long-term use, the resin bed

ENrich Instruction Manual 93.3 Frit ReplacementThe top frit may need to be replaced after extensive column use or if increasing backpressures are

10 ENrich Instruction Manual 4. Raise the adaptor a few millimeters by slowly by turning the adjusting nut counterclockwise (Figure 5).Fig. 5. Rais

ENrich Instruction Manual 117. Stop the pump. Plug the bottom of the column. Set the column upright in a beaker. Remove the tubing and fitting fr

12 ENrich Instruction Manual 13. Lower the adaptor to the top of the bed by turning the adjusting nut clockwise (Figure 8). Fig. 8. Lower the adapt

ENrich Instruction Manual 13Section 4: Product Information Catalog Number Product Description780-0001 ENrich Q 5 x 50 Column780-0003 ENrich Q 10

Life ScienceGroupSig 121310027324 Rev E US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austr

Table of ContentsSection 1: Characteristics of the ENrich Ion Exchange (IEX) Columns ... 11.1 Introduction ...

ENrich Instruction Manual 1Section 1: Characteristics of the ENrich Ion Exchange Columns 1.1 IntroductionENrich prepacked ion exchange columns me

2 ENrich Instruction Manual Table 1. Column characteristics.Q SColumn dimensions(diameter x height, mm)5 x 50 10 x 100 5 x 50 10 x 100Colu

ENrich Instruction Manual 3Section 2: Use of the ENrich Columns2.1 Preparation for Initial Use The columns are supplied in a storage solvent of 2

4 ENrich Instruction Manual Table 2. Buffers for ion exchange chromatography.Anion Exchange — ENrich QpH RangeBuffer MW pKa @ 25°CCounter-Ion pKa/°C

ENrich Instruction Manual 52.3 Sample Preparation Proper adjustment of the sample pH and ionic strength is critical for consistent and reproducib