Bio-rad Econo Gradient Pump Kits Manual do Utilizador descarregar pdf (Página 14)

4.0 TUBING SELECTION AND INSTALLATION

4.1 TUBING SELECTION

The Econo Gradient Pump may be used with most flexible tubing

having an inner diameter less than or equal to 3.2 mm (1/8”), and a

wall thickness of 1.0 mm or less, including Silicone, Tygon, and

PharMed. Silicone tubing, the most inert of the three, is suitable for

aqueous and polar solutions. Tygon is suitable for most aqueous

solutions. It will generally have the shortest lifetime of the three and

should not be used for the pumphead. PharMed is the longest lasting

of the three, and will provide the most consistent flow rate over time.

Figure 4-1 shows approximate flow rate ranges with different tubing

sizes. Flow rates above 20 ml/min may be obtained by plumbing two

channels of 3.2 mm tubing through the pump and joining them at the

output.

Very low flow rates can be achieved with tubing having ID as small as

0.51 mm. Tubing this small must be jacketed inside larger tubing and

requires an adapter. These items are included in the optional splitter

valve kit.

Figure 4-1. Approximate Flow Rate Ranges.

Tubing length and the amount of tubing prestretch have a significant

effect on both flow rate calibration and reproducibility of flow. The

PharMed tubing supplied with the Econo Gradient Pump has been

cut to a predetermined length to accommodate tubing prestretch.

(Silicon tubing in precut lengths is also available from Bio-Rad. See

ordering information in Appendix D.)

Tubing Selection

TUBING SIZE

(ID x WALL)

FLOW RATE RANGE (ml/min)

0.51 x 0.8 mm

0.8 x 0.8 mm

1.6 x 0.6 mm

3.2 x 0.8 mm

.001 .002 .005 .01

.02 .05 .10 20.20 .5 1.0 2510

ml/

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Bio-rad Econo Gradient Pump Kits Manual do Utilizador Página 14