Bio-rad Microplate Manager Software Manual do Utilizador Página 100
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Appendix
92
Percent Max Binding Assay Tutorial
1 Defining the Template
2 Setting up the Equations
3 Selecting Curve Fit and Customizing Curve Fit Plot
4 Entering Experimental Info
5 Setting up the Report
An exam
ple of how to set up an EL
ISA Assay including a standard curve and
equations to transform the absorbance data into percentage values of the maximum
bound well is given here.
Open sample data file (Percent Max Binding.mpl) with its protocol and template
C:\Program Files\Bio-Rad\Microplate Manager 6\Sample Data
1 Defining the Template
1. Click on the Template icon in the Main Toolbar
- Microplate Manager 1
- 6 Software 1
- Bio-Rad Technical Support 3
- Table of Contents 4
- Introduction 9
- Menu Bars and Icons 10
- File Menu 11
- Window Menu 12
- Minimum Requirements 13
- Entering Filters 15
- Quick Start 18
- Instrument Controls 19
- Incubator Controls 20
- Shaker Controls 20
- Reading Microplates 21
- Data and Protocol Files 22
- Opening Files 23
- Saving Files 23
- Data AutoSave 24
- Importing and Exporting Data 24
- Entering Data Manually 25
- Exporting Data or Reports 26
- Exporting Data 26
- Exporting Reports 27
- TSE, NSP) 28
- BSE Custom Screening Report 29
- Setting Up a Protocol 30
- Setting up a Protocol 31
- Setting Up A Template 33
- Entering Equations 34
- Entering Dilutions 35
- Saving the Protocol 39
- Analyzing Data 40
- Using a Standard Curve 40
- Linear Fit 41
- Zero-intercept Linear 42
- Semi-log Fit 43
- Log/log Fit 44
- Quadratic 44
- Point to Point 47
- Cubic Spline 47
- Equations and Calculations 50
- Analyzing Data Using Cutoffs 53
- + for addition 54
- / for division 54
- Analysis Report 56
- Running Kinetic Assays 60
- Analyzing Kinetics Data 61
- Kinetic Zoom Plots 62
- Viewing Raw Data at 63
- Kinetic Time Points 63
- Defining Kinetic Parameters 64
- Producing Reports 65
- Printing Reports 69
- Customizing Reports 71
- Defining the Template 73
- Setting up the Equations 74
- Entering Experimental info 75
- Dilution Data Assay Tutorial 76
- 2 Setting up the Dilutions 78
- Curve Fit Plot 81
- 4 Entering Experimental info 95
- 5 Setting up Report 96
- 1 Defining the Template 100
- 2 Setting up the Equations 101
- Fit Plot 102
- Appendix 103
- 4 Entering Experimental Info 107
- 5 Setting up the Report 108
- Life Science 112
- Bio-Rad 112
- Laboratories, Inc 112
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