Bio-rad Microplate Manager Software Manual do Utilizador descarregar pdf (Página 101)

Appendix

• Blanks are defined as #. The average of these # wells will be

subtracted from all the wells.

• NSB are non-specific binding wells.

• MAX is for the maximum binding well (i.e. the Bo well).

• S01 to S21 are all of the unknown samples to be analyzed.

2 Setting up the Equations

1. Click Equation Editor icon in the Template Toolbar.

2. Choose % MAX from Name popup to view equations to be applied

sequentially to data

• @ - # — Subtracts mean of blank (#) wells

• @ - NSB — Subtracts mean of non specific binding (NSB) wells

• @ / MAX — Divides by mean of maximum binding (MAX) wells

• @ * 100 — Multiplies by 100 to convert to % bound

1 2 ... 96 97 98 99 100 101 102 103 104 105 106 ... 111 112

Microplate Manager 1
6 Software 1
Bio-Rad Technical Support 3
Table of Contents 4
Introduction 9
Menu Bars and Icons 10
File Menu 11
Window Menu 12
Minimum Requirements 13
Entering Filters 15
Quick Start 18
Instrument Controls 19
Incubator Controls 20
Shaker Controls 20
Reading Microplates 21
Data and Protocol Files 22
Opening Files 23
Saving Files 23
Data AutoSave 24
Importing and Exporting Data 24
Entering Data Manually 25
Exporting Data or Reports 26
Exporting Data 26
Exporting Reports 27
TSE, NSP) 28
BSE Custom Screening Report 29
Setting Up a Protocol 30
Setting up a Protocol 31
Setting Up A Template 33
Entering Equations 34
Entering Dilutions 35
Saving the Protocol 39
Analyzing Data 40
Using a Standard Curve 40
Linear Fit 41
Zero-intercept Linear 42
Semi-log Fit 43
Log/log Fit 44
Quadratic 44
Point to Point 47
Cubic Spline 47
Equations and Calculations 50
Analyzing Data Using Cutoffs 53
+ for addition 54
/ for division 54
Analysis Report 56
Running Kinetic Assays 60
Analyzing Kinetics Data 61
Kinetic Zoom Plots 62
Viewing Raw Data at 63
Kinetic Time Points 63
Defining Kinetic Parameters 64
Producing Reports 65
Printing Reports 69
Customizing Reports 71
Defining the Template 73
Setting up the Equations 74
Entering Experimental info 75
Dilution Data Assay Tutorial 76
2 Setting up the Dilutions 78
Curve Fit Plot 81
4 Entering Experimental info 95
5 Setting up Report 96
1 Defining the Template 100
2 Setting up the Equations 101
Fit Plot 102
Appendix 103
4 Entering Experimental Info 107
5 Setting up the Report 108
Life Science 112
Bio-Rad 112
Laboratories, Inc 112

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