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Analyzing Data Using Cutoffs

Reference can be made to standard or sample IDs that have been defined in the

Template setup

• Standards: enter ! followed by concentration

• Unknowns: enter identifier

• Blanks: enter #

In all Cutoff equations, Microplate Manager 6.0 uses the averaged value of that

group.

Examples of valid expressions:

• U01

• (U01+.1)/2

• (!100-!10)*2

Cutoff Examples for Assays with Controls

Positive & Negative Controls – positive (POS) & negative (NEG) control well(s)

defined on template

• Low Cutoff: NEG

• High Cutoff: POS

% B/Bo – non-specific binding blank (NSB) & maximum or Bo well(s) (MAX)

defined on template

• Low Cutoff: NSB

• High Cutoff: MAX

% Calibrator –blank (#) & calibrator (CALB) well(s) defined on template

• Low Cutoff: #

• High Cutoff: CALB

% Control - non-specific binding blank (NSB) & control well(s) (CNTRL) defined

on template

• Low Cutoff: NSB

• High Cutoff: CNTRL

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Bio-rad Microplate Manager Software Manual do Utilizador Página 55