Bio-rad Microplate Manager Software Manual do Utilizador Página 46
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Analyzing Data Using a Standard Curve
38
The 4-parameter method is usually preferred over the Log/Logit method, because the
results for the 4-parameter method are more accurate.
In both the Log/Logit and the 4-Parameter plots, the concentration axes are displayed
on the log scale. The parameters A, B, C, D, the chi
2
values of the standard curve are
shown below the plot.
- Microplate Manager 1
- 6 Software 1
- Bio-Rad Technical Support 3
- Table of Contents 4
- Introduction 9
- Menu Bars and Icons 10
- File Menu 11
- Window Menu 12
- Minimum Requirements 13
- Entering Filters 15
- Quick Start 18
- Instrument Controls 19
- Incubator Controls 20
- Shaker Controls 20
- Reading Microplates 21
- Data and Protocol Files 22
- Opening Files 23
- Saving Files 23
- Data AutoSave 24
- Importing and Exporting Data 24
- Entering Data Manually 25
- Exporting Data or Reports 26
- Exporting Data 26
- Exporting Reports 27
- TSE, NSP) 28
- BSE Custom Screening Report 29
- Setting Up a Protocol 30
- Setting up a Protocol 31
- Setting Up A Template 33
- Entering Equations 34
- Entering Dilutions 35
- Saving the Protocol 39
- Analyzing Data 40
- Using a Standard Curve 40
- Linear Fit 41
- Zero-intercept Linear 42
- Semi-log Fit 43
- Log/log Fit 44
- Quadratic 44
- Point to Point 47
- Cubic Spline 47
- Equations and Calculations 50
- Analyzing Data Using Cutoffs 53
- + for addition 54
- / for division 54
- Analysis Report 56
- Running Kinetic Assays 60
- Analyzing Kinetics Data 61
- Kinetic Zoom Plots 62
- Viewing Raw Data at 63
- Kinetic Time Points 63
- Defining Kinetic Parameters 64
- Producing Reports 65
- Printing Reports 69
- Customizing Reports 71
- Defining the Template 73
- Setting up the Equations 74
- Entering Experimental info 75
- Dilution Data Assay Tutorial 76
- 2 Setting up the Dilutions 78
- Curve Fit Plot 81
- 4 Entering Experimental info 95
- 5 Setting up Report 96
- 1 Defining the Template 100
- 2 Setting up the Equations 101
- Fit Plot 102
- Appendix 103
- 4 Entering Experimental Info 107
- 5 Setting up the Report 108
- Life Science 112
- Bio-Rad 112
- Laboratories, Inc 112
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