Bio-rad BioLogic LP System Manual do Utilizador Página 8
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1.0 INTRODUCTION
1.1 OVERVIEW
The BioLogic LP low-pressure, gradient chromatography system is designed for the purification of proteins,
peptides, and other biomolecules where recovery of biological activity is of primary concern.
The BioLogic LP Controller is microprocessor controlled, with easy-to-use front panel controls and menu-dri-
ven software for manual operation, system setup, method editing and run operations.
The flexible control architecture allows the seamless integration of a wide variety of configurations with other
Bio-Rad and non-Bio-Rad components to meet your purification requirements.
Figure 1. BioLogic LP System
1
Introduction
SV-5 BUFFER SELECT
MV-6 INJECT VALVE
UV OPTICS MODULE
CONDUCTIVITY FLOW CELL
PROPORTIONING VALVE
AND MIXER MODULE
MODEL 1327
CHART RECORDER
BIOLOGIC LP
CONTROLLER
SYSTEM RACK
MODEL 2128 FRACTION
COLLECTOR
ECONO COLUMN
SV-3 DIVERTER VALVE
SV-3 BYPASS VALVE
- BIOLOGIC LP 1
- CHROMATOGRAPHY SYSTEM 1
- TABLE OF CONTENTS 2
- GETTING STARTED QUICKLY 6
- 1.0 INTRODUCTION 8
- 1.2 FEATURES 9
- 1.3 UNPACKING 10
- Table 1. (continued) 11
- 2.0 DESCRIPTION OF COMPONENTS 13
- Table 3 14
- Table 3. (continued) 15
- 2.3 UV DETECTOR 17
- 2.4 CONDUCTIVITY DETECTOR 18
- 2.5 BIO-RAD SYSTEM OPTIONS 19
- Threshold 20
- Next tube 2 20
- Draining 20
- 3.0 SYSTEM CONNECTIONS 24
- Figure 10. Rack Assembly 25
- System Connections 27
- 4.0 SYSTEM PLUMBING 29
- Figure 12. Luer Fittings 30
- PLATEN ADJUST 31
- LOCK-RING 31
- LUER FITTING 31
- 4.3 PLUMBING THE SYSTEM 33
- Figure 14. System Plumbing 34
- 4.4 PURGING THE SYSTEM 37
- 5.0 SYSTEM OPERATION 38
- Table 5. (continued) 39
- Front Panel Controls 39
- 5.2 MANUAL MODE 41
- System Operation 42
- Table 6. (continued) 43
- Manual Mode Operation: Pump 43
- Table 6. (continued) 44
- Table 6 45
- Table 7 45
- Table 7. (continued) 46
- Table 8 48
- Table 9 49
- Table 9. (continued) 50
- Table 10 51
- Table 10. (continued) 52
- Table 11 53
- Table 11. (continued) 54
- Table 12 55
- 5.3 PROGRAMMING MODE 56
- Table 13 58
- Table 13. (continued) 59
- Figure 22. List of Methods 59
- 5.3.2 Creating a New Method 60
- Table 15 63
- Table 15. (continued 64
- Table 16 65
- Program Mode’s Pump Table 65
- Table 16. (continued) 66
- Table 17 69
- Table 17. (continued) 70
- Table 18 71
- Table 19 72
- Table 20 74
- Table 20. (continued) 75
- Table 21 77
- Table 21. (continued) 78
- Table 22 80
- Program Mode’s Alarm Table 80
- Table 22. (continued) 81
- 5.3.7 Entering Method Names 82
- 5.4 RUN MODE 83
- 5.4.1 Starting a Run 83
- Table 24. (continued) 84
- Run Mode: Starting a Run 84
- 5.4.2 Run in Progress 86
- Table 24 87
- Table 25 88
- Run Mode: Run Hold 88
- Table 26 89
- Run Mode: Run Pause 89
- 6.1 CLEANING AND STORAGE 92
- 6.2 PUMP CALIBRATION 92
- 6.2.1 Nominal Calibration 93
- 6.2.2 User Calibration 93
- Figure 32. UV Optics Module 96
- 6.6 TROUBLESHOOTING 97
- APPENDIX A. SPECIFICATIONS 99
- WARRANTY INFORMATION 101
- ORDERING INFORMATION 102
- 4006072 Rev D 106
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