Bio-rad Ligation and Transformation Module Manual do Utilizador Página 2
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- Índice
- MARCADORES
- Biotechnology Explorer 1
- Ligation and Transformation 1
- Instruction Manual 1
- Table of Contents 3
- Introduction 5
- Activity? 6
- Transformation Protocol? 7
- E. coli 9
- Kit Inventory Checklist 10
- Safety Issues 13
- Background 14
- DNA Ligation 17
- Sticky end digestion 19
- Blunt end digestion 20
- PCR Fragment 21
- Products of Ligation 23
- Transformation 24
- Chamber for cells 26
- Metal electrodes 26
- Minipreps of Plasmid DNA 28
- Ligation – Quick Guide 30
- Transformation – Quick 32
- Experimental Procedure for 34
- 37°C incubator 35
- Ligation Reaction 36
- Transformation Laboratory 37
- Student Ligation Protocol 40
- Ligation of gene of interest 49
- Control plasmid 49
- Appendix A 50
- Miniprep 50
- (catalog #732-6400EDU) 51
- Appendix B 52
- Bgl II Enzyme 52
- Legal Notices 56
- Trademarks 56
- Life Science 60
- Bio-Rad 60
- Laboratories, Inc 60
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