Bio-rad Ligation and Transformation Module Manual do Utilizador descarregar pdf (Página 9)

When Activity to Complete Duration

Immediately following Transform

E. coli

with ligation 1 h

ligation or during the mixture and plate bacteria on

next laboratory activity LB Amp IPTG agar plates

Incubate transformed bacteria 16+ h

at 37°C

Next day after the Analyze results 0.5 h

transformation step Grow bacterial colony in LB Amp 16+ h

broth for miniprep

Day after growing Perform miniprep plasmid 1 h

bacterial culture for purification to isolate

miniprep plasmid carrying insert

Next laboratory activity Digest plasmid DNA with 1 h

Bgl II restriction enzyme

Analyze digest by agarose gel 1 h

electrophoresis

Prepare the DNA insert for 0.5 h

sequencing

Note: Bolded steps use reagents from the Ligation and Transformation

module.

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Bio-rad Ligation and Transformation Module Manual do Utilizador Página 9