Bio-rad Ligation and Transformation Module Manual do Utilizador descarregar pdf (Página 55)

Restriction enzyme digestion analysis of plasmid DNA. Circular plasmid DNA purified from

bacterial minipreps is isolated and digested with Bgl II, a restriction enzyme producing at least

two linearlized fragments — vector DNA and PCR fragment (lane 2). These fragments can be

visualized using agarose gel electrophoresis. If the PCR fragment contains a Bgl II restriction

site, three DNA bands may be observed (lane 3). A 500 bp molecular weight ruler is shown in

lane 1.

BgI II

PCR

Fragment

PCR Fragment

BgI II

Bgl II

Digest

1 2 ... 50 51 52 53 54 55 56 57 58 59 60

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Bio-rad Ligation and Transformation Module Manual do Utilizador Página 55