Bio-rad VINEO™ Brettanomytest PCR Kit Manual do Utilizador descarregar pdf (Página 2)

TABLE OF CONTENTS

1 - INTRODUCTION

2 - PRINCIPLE BEHIND THE VINEO™ Brettanomytest PCR Kit

3 - KIT COMPOSITION

4 - VALIDITY AND PRESERVATION

5 - REQUIRED EQUIPMENT THAT IS NOT PROVIDED

6 - PRECAUTIONS AND RECOMMENDATIONS

7 - SAMPLING AND TRANSPORT OF SAMPLES

8 - DNA EXTRACTION

9 - REAL-TIME PCR

10 - ANALYSIS OF THE DATA

11 - INTERPRETATION OF THE RESULTS

12 - CONFIRMATION PROTOCOL FROM ISOLATED COLONIES

13 - TEST PERFORMANCE AND VALIDATIONS

APPENDIX A - PCR AMPLIFICATION REAGENT MIXTURE

PREPARATION TABLE

APPENDIX B - PLATE SETUP FOR THE Chromo4™ OR THE CFX96™

THERMOCYCLERS

APPENDIX C - PLATE SETUP FOR THE MiniOpticon™

THERMOCYCLER

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Bio-rad VINEO™ Brettanomytest PCR Kit Manual do Utilizador Página 2