Bio-rad VINEO™ Brettanomytest PCR Kit Manual do Utilizador descarregar pdf (Página 6)

Consumables:

• For the plates, PCR tubes and stop caps:

- White plates of 96 "Low-profile" wells, ref. Bio-Rad: MLL-9651

- White plates of 48 "Low-profile" wells, ref. Bio-Rad: MLL-4851

- Strips of 8 tubes of 200l "Low-profile", ref. Bio-Rad: TLS-0851

- Strips of 8 flat optical stop caps for tubes or plates of 0.2 mL wells,

120 strips ref. Bio-Rad: TCS-0803

• Cones for Combitip or multi-distributor, sterile when packaged

individually

• Cones with sterile filters that are adaptable to 10 µL, 20 µL, 100 µL,

200 µL and 1000 µL micro-pipettes

• 2mL and 5mL sterile tubes

• Non-powdered latex or nitrile gloves

• Sterile distilled water

• 5% Chlorine Bleach

• Decontaminating agent such as DNA AWAY

or RNAse AWAY

6 - PRECAUTIONS AND RECOMMENDATIONS

• This trial must be carried out by staff who have had adequate

training.

• Result quality depends on scrupulous compliance with Laboratory

Good Practice, in particular in the area of PCR:

- The equipment (pipettes, tubes etc.) must no go from one work

station to another.

- It is indispensable to use a positive and a negative control for each

series of amplification reactions.

- Do not use the reagents beyond their expiry date.

- Subject the reagents in the kit to a vortex movement using the

apparatus provided for this purpose before using them to work

with homogeneous solutions.

- Check the exactness and the precision of the pipettes as well as

the proper functioning of the instruments.

- Change gloves regularly and as soon as you suspect that they may

have been contaminated.

- Clean work surfaces regularly with a 5% solution of chlorine bleach

and another agent such as DNA AWAY

or RNase AWAY

- Wear non-powdered gloves so as not to leave fingerprints on the

optical film used to seal the micro-plates. Do not write on the PCR

tube stoppers. In both cases, recording the data by the device can

be disturbed.

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Bio-rad VINEO™ Brettanomytest PCR Kit Manual do Utilizador Página 6