Bio-rad VINEO™ Brettanomytest PCR Kit Manual do Utilizador descarregar pdf (Página 5)

This test is used to detect and quantify Brettanomyces bruxellensis in the

DNA extracted from fermented drinks produced from grape and

fermenting grape musts.

3 - KIT CONTENTS

This kit contains the quantity of reagent required for 96 reactions.

4 - VALIDITY AND PRESERVATION

On reception, the kit must be stored at temperatures between +2°C and

+8°C. Each reagent stored between +2°C and +8°C can be used until the

expiry date indicated on the tube.

NB: Do not freeze the reagents.

5 - REQUIRED EQUIPMENT THAT IS NOT PROVIDED

Equipment:

• Industrial Diagnostic CFX96™ real-time PCR detection system, 96

wells, ref. Bio-Rad :359-3990

• CFX Manager™ Software, Industrial Diagnostic Edition, ref. Bio-Rad :

359-3893

• MiniOpticon™ system for real-time PCR, reaction block of 48 wells,

ref. Bio-Rad: 359-3995

• Vortex

• Optionally: centrifuge with rotor for plates or strips (max. 2000 x G)

• 20 µl, 200 µl and 1000 µl micropipettes

• Multi-distributor such as combitip pipettes

Note: We recommend using an uninterruptible power supply with the

thermocycler.

Stoppers Designation Reagent Volume

Pink Fluorescent probes Fluorescent probes 1 tube (0.55 mL)

White Amplification mix Amplification mix 2 tubes (2 x 2.2 mL)

Green Negative PCR control Negative PCR control 1 tube (0.5 mL)

Red Positive Qs PCR control Positive Qs PCR control 1 tube (0.5 mL)

TAKING

THE SAMPLE

DNA EXTRACTION

VINEO™ Extract

DNA Kit

(Ref.: 354-8100)

DNA

AMPLIFICATION by

PCR IN REAL TIME

VINEO™

Brettanomytest PCR

Kit (Ref.: 354-8101)

ANALYSIS &

INTERPRETATION

Opticon Monitor™

CFX Manager™ IDE

1 2 3 4 5 6 7 8 9 10 ... 55 56

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