Bio-rad S3™ Cell Sorter Manual do Utilizador descarregar pdf (Página 31)

Instruction Manual | 21

3.2 Control Panel

Table 20. Instrument controls and their functions.

Button Name Function

Start Sample Starts sample acquisition. If a data ﬁle is loaded and it has been saved,

Acquisition the data will clear and acquisition will start

Stop Sample Stops acquisition and sample ﬂow. Regions, gates, and annotations may

Acquisition be modiﬁed before saving the FCS ﬁle

Start Sorting Starts sorting. If a data ﬁle is loaded and it has been saved, the data will

clear and sorting will start

Stop Sorting Stops sorting and sample ﬂow. Regions, gates, and annotations may be

modiﬁed before saving the FCS ﬁle

Clear Acquired Data Clears acquired data. All plots and statistics will be refreshed.

Pause Sample Pauses sample acquisition and sample ﬂow. The data at this point may be

appended (by the Resume function) or saved as is. Saving after pausing will

disable the Resume function. To remove sample tube, unlock the touchscreen

locking system located above the loading stage after pausing acquisition

Resume Sample Resumes acquisition when a sample has been paused. Resuming will

append the paused FCS ﬁle and the time paused can be seen on a time

histogram. If you have removed the sample tube it must be returned and

relocked before acquisition can be resumed

Eject Sample Tube Depressurizes and unlocks the sample. The loading stage can be moved

into wash position to access sample. The touchscreen locking system can

also be used to unlock the sample

Adjust Sample Starts or stops agitation of the sample. There are three levels of agitation:

Agitation low, high, and off. Click this button once to set to low, twice to set to high,

and three times to turn off agitation

Auto Save When checked, automatically prompted to save a data ﬁle after

acquisition stops.

Target Event Rate Allows selection of target event rate for sample acquisition or use of preset

sample rates low/med/high based on sample concentration

Limit When checked, stops data acquisition after the number of events entered

has been reached

Gate Limit When checked, data acquisition stops after the number of events entered

has been reached within the selected gate

Cycle Mode When checked, automatically refreshes the data displayed according to

the entered time. The acquired data set continues to accumulate in the ﬁle

Sample Chamber Turns the sample chamber light on and off

Light

Enable/Disable Sort Illuminates entire sort collection chamber for easy viewing of deﬂection

Chamber Light plates, sort stream, and collection vessels

Enable/Disable Enables or disables temperature control of the sample station and sort

Temperature Control collection vessel

Biosafety Indicates the status of the biosafety system and gives information on ﬁlter life.

System Status If the biosafety system loses connection, cannot connect, or if the ﬁlter life is

at 0% an error will occur and not allow the user to sort or acquire.

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