Bio-rad S3™ Cell Sorter Manual do Utilizador descarregar pdf (Página 48)

38 | S3 and S3e Cell Sorters

Getting Started

4.4.2 Side Streams

Following droplet setup, the side streams are adjusted to align with the sort tube positions and to

ensure they are not fanning. The streams may be viewed by clicking View Streams on the Home

tab. The View Streams window displays a charge phase and defanning slider bar (Figure 38).

Users can manually adjust the charge phase and defanning values by moving the slider bar. The

Enable Test Pattern button should be selected in order to apply a charge to the stream.

Users with administrator privileges may access

and modify these settings in the Administrator

tab. For more information, refer to Section 3.3,

Administrator Tab Toolbar.

CAUTION! Optimal side stream

settings are calculated automatically

after performing a quality control

procedure. Adjusting any of these

values will change the optimal

settings and will affect the side

streams and sort.

Fig. 37. Droplet Monitor window.

Fig. 38. View Streams window with charge

phase and defanning slider bars highlighted.

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Bio-rad S3™ Cell Sorter Manual do Utilizador Página 48