Bio-rad S3™ Cell Sorter Manual do Utilizador descarregar pdf (Página 32)

22 | S3 and S3e Cell Sorters

ProSort Software

Fig. 17. Administrator tab toolbar.

Table 20. Instrument controls and their functions (continued).

Button Name Function

Fluidics System Indicates the status of the ﬂuidics system and run time information. When

Status the ﬂuidics system is down to an hour of run time, the ﬂuidics status

window will automatically open to show which container needs attention.

If the run time reaches 5 min, the ﬂuidics will automatically shut down

Toggle 488 nm Controls the 488 nm laser shutter. The shutter is interlocked for safety. When

Laser Shutter this button is grayed out, either the laser is off or the interlock is open

Toggle 561 or 640 nm Controls the 561 or 640 nm laser shutter. The shutter is interlocked for safety.

Laser Shutter When this button is grayed out, either the laser is off or the interlock is open

Adjust Laser The dropdown arrows next to each laser adjust the laser power. Actual

mWatt Power power is shown by hovering over the 488 nm, 561 nm, and 640 nm labels

Table 21. PMT controls and their functions.

Button Name Function

Editable The name for each parameter is editable and will be saved with the

parameter acquired FCS data. The edited parameter name will be applied to all

name plot axes, compensation controls, and ﬁlter conﬁgurations

PMT Voltage Adjusts the PMT voltage for the given detector

Select trigger The trigger parameter is used to alert the system to the presence of

parameter an event over the threshold. The default for this is Forward Scatter

Adjust threshold Threshold is a percentage of the trigger signal. The default value is 1.0%

setting

3.3 Administrator Tab Toolbar

The administrator tab is available only for administrator users. To change a user’s privileges,

refer to Section 3.3.5, Managing Users.

The toolbar for the administrator tab (Figure 17) consists of:

n

System and User Control — edit system controls and manage users

Table 22 describes the buttons in this section.

IMPORTANT! Adjusting any of these settings will impact the overall performance of

the system. Only knowledgeable users should make any changes to these settings.

All changes made here will apply globally to the system.

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