Bio-rad S3™ Cell Sorter Manual do Utilizador descarregar pdf (Página 84)

74 | S3 and S3e Cell Sorters

Additional Software Features

7.4 Clean System

In between samples or users, cleaning can be done at low or high pressure. High pressure

cleaning will cause the system to stop maintaining droplets and a QC procedure must be run

before sorting. If cleaning between samples, the low pressure option is recommended. The

ﬂuidics lines will be ﬂushed thoroughly with cleaning solution, similarly to the cleaning protocol

used during the shutdown or logout procedure.

To clean the system:

1. Fill a 5 ml tube with a minimum of 3 ml of cleaning solution.

2. Load tube into the sample station and move in to run position.

3. Click the Set Up and Maintenance tab.

4. Click Clean System.

The cleaning protocol will automatically begin. A prompt will appear to warn the user that the

clean function will begin and that the contents within the sample tube will be used rapidly.

Fig. 78. Cleaning protocol warning box.

Fig. 79. Clean System modes.

Fig. 80. Warning for high pressure cleaning protocol.

5. Select the cleaning mode to run (Figure 79).

6. Click Yes to proceed with mode (Figure 80).

7. Move the loading stage into wash position (Figure 81).

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Bio-rad S3™ Cell Sorter Manual do Utilizador Página 84