Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 125)

Priming and Purging the Systems

Instrument Guide | 123

5. In the System Pump dialog box, select Priming on the Mode dropdown list.

Note: When in Priming mode, the sample inject valve switches the system

pump flow to waste (W2). After the process is completed, the sample inject

valve switches the system pump to manually load the column through the

loop.

By default, all ports are closed. The Closed radio button is selected.

6. Select Q4 as the port to open and prime.

7. Insert a 30 ml syringe into the priming port on system pump A.

8. Turn the priming port valve counter-clockwise one full turn to open the seal.

9. Slowly withdraw the

plunger to withdraw liquid from the port

until

air no

longer enters the syringe (approximately 10 ml)

and the tubing is filled with

liquid.

10. Turn the priming port valve clockwise to close the port.

11. Remove the syringe from the priming port and discard the liquid into waste.

12. Repeat steps 7–11 for ports Q3–Q1.

13. To prime system pump B, select port Q3 perform steps 7–11.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 125