Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 255)

UV or UV/Vis Detector

Instrument Guide | 253

UV or UV/Vis Detector

Symptom Possible Cause Possible Solution

The UV baseline is

unstable or noisy.

The UV baseline shows

a reproducible zig-zag

or sawtooth trace.

Air bubbles are trapped in the flow

cell.

Note: Always degas buffers

and solutions before use.

 Degas buffers by stirring

vigorously under vacuum for

approximately 20 min.

Important: Use a

heavy-wall, side-arm

Erlenmeyer flask, as

standard flasks may

implode under vacuum.

 Plumb the detector with the

column outlet connected to

the bottom of the flow cell.

This forces air bubbles to rise

to the top of the flow cell and

dissipate.

 Use the 40 psi backpressure

regulator supplied with the

NGC system to remove most

air bubbles. Place it directly

after the conductivity flow cell

and run buffer for several

minutes.

If the chosen column cannot be

run with the backpressure

regulator, and air bubbles are a

continuing problem (for example,

during some affinity

chromatography steps), use the

2 mm path length flow cell. The

straight line flow of the flow cell

makes it easier to remove

bubbles.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 255

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