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Mixer

Instrument Guide | 6 3

Detailed Attributes

The mixer is composed of a base motor, a stirring plate, and a hollow mixer barrel in

which the buffers are mixed. An optional mixer barrel extender is available to adapt

the mixer volume to the flow rate used. Bio-Rad offers several different mixer

chamber sizes for different flow rates. Table 2 and Table 3 list the recommended

barrel sizes for different flow rates for each type of system pump. To change the

barr

els, see Replacing the Mixer Components on page 186.

Tip: For liquids that are difficult to mix, use a mixer barrel with a higher

capacity.

Table 2. Flow rates of supported mixer barrels for the F10 pumps

Mixer Barrel Size Flow Rate Range, ml/min

Without buffer blending valve

(gradient)

With buffer blending valve

263 μl (base) .01–1.00 0.1–1.99

750 μl 1.00–9.99 2.00–5.99

2 ml 10.00–20.00 6.00–11.99

5 ml n/a 12.00–20.00

Table 3. Flow rates of supported mixer barrels for the F100 pumps

Mixer Barrel Size Flow Rate Range, ml/min

Without buffer blending valve

(gradient)

With buffer blending valve

750 μl (base) 1.00–4.99 <5.00

2 ml 5.00–14.99 5.00–11.99

5 ml 15.00–39.99 12.00–39.99

12 ml 40.00–100.00 40.00–80.00

1 2 ... 60 61 62 63 64 65 66 67 68 69 70 ... 305 306

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Bio-rad ChromLab™ Software Manual do Utilizador Página 65