Bio-rad ChromLab™ Software Manual do Utilizador Página 269
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Plumbing NGC Systems
Instrument Guide | 267
To plumb and prime the system pump wash lines
1. Mount a tube holder to the right or left side of the instrument in either the upper
or lower rack slots.
2. Fill a 50 ml tube with 20% isopropanol (IPA) and insert it in the tube holder.
Tip: You can use 20% ethanol as an alternative.
3. Connect inlet tubing to the pump wash inlet port (left rear port) on
system pump A.
4. Immerse the other end of the pump A wash inlet tubing in the tube of 20% IPA.
5. Connect the pump head wash priming tubing to the outlet (right rear port) on
system pump B.
6. Connect a 1/4-28 female to female luer adaptor to the pump head wash
priming tubing.
7. Connect a 1/4-28 union to a second inlet tubing. You will use the inlet tubing as
the outlet tubing for system pump B.
Tip: Set the inlet tubing with the attached union aside. Keep it close by, as
you will attach it to the pump head wash priming tubing on step 12.
- Chromatography Systems 1
- Software 1
- Instrument Guide 1
- NGC™ Chromatography 3
- Systems and 3
- Instrument Guide 7
- Instrument Guide 9
- Table of Contents 10
- Instrument Guide 11
- 1 Introduction 13
- (on the NGC Quest 14
- Plus system) 14
- Main Features 15
- Site Requirements 16
- Environment 17
- Bench Space 17
- Safety Requirements 18
- Finding Out More 19
- 1 20
- 2 The NGC Instrument 21
- 2 22
- Instrument Guide 23
- Front View 24
- Instrument Guide 25
- Back View 26
- Right Side View 27
- Left Side View 28
- System Pumps 31
- Detailed Attributes 32
- System Pump LEDs 32
- How the System Pumps Function 32
- Pump Priming Port 33
- Piston Wash System 34
- Sample Pump 35
- Sample Pump LEDs 36
- How the Sample Pump Works 36
- Instrument Guide 37
- Sample Inject Valve 39
- Port Function 40
- Sample Inject Valve LEDs 41
- Valve Position Explanation 42
- Buffer Blending Valve LEDs 46
- Buffer Blending Mode 47
- Gradient Mode 47
- Inlet Valves 48
- Inlet Valve 49
- Ports on the Inlet Valves 50
- Inlet Valve LEDs 51
- How the Inlet Valves Work 52
- Outlet Valve 53
- Ports on the Outlet Valve 54
- Outlet Valve LEDs 55
- How the Outlet Valve Works 56
- Column Switching Valve LEDs 60
- Instrument Guide 61
- Instrument Guide 63
- Detectors 66
- Instrument Guide 67
- Single-Wavelength UV Detector 68
- Instrument Guide 69
- Conductivity Detector 71
- How They Work 72
- The Backpressure Regulator 73
- Ports on the pH Detector 75
- How the pH Detector Works 76
- Instrument Guide 77
- Pressure Rating 78
- Mounted 78
- Sample Injection Loops 79
- Fittings 80
- LEDs and LED Screens 81
- Air Sensors 82
- Instrument Guide 83
- Air Sensor LEDs 84
- Pressure Sensors 85
- Temperature Sensor 85
- NGC Touch Screen 86
- Buffer Tray 87
- Product Configurations 88
- Discover series 89
- 3 Preparing the Instrument 91
- Module Review 92
- Instrument Guide 93
- NGC Systems Accessories Kit 94
- NGC Scout Accessories Kit 95
- NGC Discover Accessories Kit 95
- Buffer Bottles 96
- Waste Bottles 96
- Instrument Guide 97
- Preparing the Detectors 99
- Changing the Mixer Barrel 100
- Barrel Size Flow Rate, ml/min 101
- F10 Pump F100 Pump 101
- (air sensor to pump inlet) 103
- To activate the air sensors 105
- Instrument Guide 107
- Instrument Guide 109
- Instrument Guide 111
- Instrument Guide 113
- Trigger wire 115
- Positive 115
- Starting the NGC Instrument 116
- Instrument Guide 117
- To verify the plumbing path 119
- To prime the system pumps 121
- To purge the system pumps 122
- Instrument Guide 123
- Priming the NGC Scout System 124
- Instrument Guide 125
- Purging the NGC Scout System 126
- Instrument Guide 127
- Priming NGC Discover Systems 128
- Instrument Guide 129
- Instrument Guide 131
- To prime the sample pump 132
- Instrument Guide 133
- Instrument Guide 135
- Instrument Guide 137
- To purge the sample pump 138
- Instrument Guide 139
- Instrument Guide 141
- Preparing the Columns 142
- Instrument Guide 143
- UV monitor 144
- Washing the Columns 145
- Calibrating pH 147
- To zero the pressure 148
- Instrument Guide 149
- A Maintaining the Instrument 151
- Storing the NGC Instrument 153
- Interval Component Action 154
- As Needed 156
- Component Solution to use 157
- System and Sample Pumps 158
- Pump Head Assembly 159
- Piston Wash Assembly 160
- Disassembling the Pumps 161
- Instrument Guide 163
- To clean the pump heads 164
- Instrument Guide 165
- Instrument Guide 167
- To replace the pump head seal 169
- Removing the Wash Housing 171
- Removing the Piston 172
- Instrument Guide 173
- Instrument Guide 175
- Reassembling the Pumps 177
- Installing the Wash Housing 178
- Instrument Guide 179
- Installing the Pump Heads 180
- Instrument Guide 181
- Instrument Guide 183
- Instrument Guide 185
- Conditioning New Seals 187
- Instrument Guide 189
- Disassembling the Mixer 190
- Instrument Guide 191
- To change the O-rings 193
- To clean the mixer body 195
- Reassembling the Mixer 196
- Instrument Guide 197
- The Detectors 198
- Instrument Guide 199
- Instrument Guide 201
- Instrument Guide 203
- Instrument Guide 205
- Instrument Guide 207
- Back of LED Board 209
- Front of LED Board 209
- To reset lamp time details 210
- Instrument Guide 211
- 6. Remove the lamp: 213
- Instrument Guide 215
- Instrument Guide 217
- Storing the pH Electrode 219
- Cleaning the pH Electrode 219
- Replacing the pH Probe 220
- Instrument Guide 221
- Other Components 222
- Instrument Guide 223
- Fourth expansion tier 224
- Previously attached 224
- For example: 225
- Instrument Guide 227
- Task: Refer to the section: 228
- Instruments 229
- Bottom of Module 231
- Top of Module 231
- Instrument Guide 233
- Instrument Guide 235
- Cutting Replacement Tubing 236
- Instrument Guide 237
- Instrument Guide 239
- Instrument Guide 241
- Instrument Guide 243
- Instrument Guide 245
- B Troubleshooting the 247
- Instrument 247
- Instrument Guide 249
- Perform CIP to clean the 250
- Replace the pump seals 250
- Replace the check valves 250
- Instrument Guide 251
- Replace check valves 252
- Instrument Guide 253
- UV or UV/Vis Detector 255
- Use the lamp usage values 256
- See Replacing the Single 256
- See Replacing the Multi 256
- Instrument Guide 257
- Recalibrate both system 258
- Instrument Guide 259
- Conductivity Monitor 260
- Instrument Guide 261
- Buffer Blending Valve 262
- Column Switching Valve 262
- External Devices 263
- C Plumbing the System 265
- Plumbing NGC Systems 266
- Plumbing All NGC Systems 267
- System Pumps Wash Flow 268
- Sample Pump Wash Flow 268
- Instrument Guide 269
- To plumb the sample pump 270
- Instrument Guide 271
- C 272
- Plumbing the NGC Quest System 273
- To plumb the system pumps 275
- Plumbing the NGC Scout System 276
- Instrument Guide 277
- To plumb the pH valve 278
- Instrument Guide 279
- Instrument Guide 281
- Tubing color Port Solution 282
- Instrument Guide 285
- Plumbing Sample Inlet Valves 287
- Plumbing Outlet Valves 288
- Instrument Guide 289
- D Connecting the C-96 291
- Instrument Guide 293
- To prepare the tubing 294
- Autosampler 295
- Sample Inject 295
- Instrument Guide 297
- E Regulatory Information 299
- 5 ml F10 2,650 psi 300
- 5 ml F100 1,400 psi 300
- DynaLoop™ 25 ml F100 700 psi 300
- DynaLoop 90 ml F100 270 psi 300
- FCC Warning and Notes 301
- F NGC Chromatography 303
- Systems Catalog Numbers 303
- Life Science 306
- Bio-Rad 306
- Laboratories, Inc 306
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