Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 104)

5 | Plumbing and Priming the NGC Chromatography Systems

102 | NGC Chromatography Systems and ChromLab Software

To plumb and prime the system pump wash lines

1. Mount a pump head wash tube holder to the right or left side of the instrument

in either the upper or lower rack slots.

2. Fill a 50 ml tube with 20% isopropanol (IPA) and insert it in the tube holder.

Tip: You can use 20% ethanol as an alternative.

3. Connect inlet tubing to the pump wash inlet port (left rear port) on

system pump A.

4. Immerse the other end of the pump A wash inlet tubing in the tube of 20% IPA.

5. Connect the pump head wash priming tubing to the outlet (right rear port) on

system pump B.

6. Locate a 1/4-28 female to female luer adaptor in the fittings kit and connect it to

the pump head wash priming tubing.

7. Connect a 1/4-28 union to a second inlet tubing. You will use the inlet tubing as

the outlet line for system pump B.

Tip: Set the inlet tubing with the attached union aside. Keep it close by, as

you will attach it to the pump head wash tubing in step 11.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 104