Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 114)

5 | Plumbing and Priming the NGC Chromatography Systems

112 | NGC Chromatography Systems and ChromLab Software

3. Immerse the open end of the waste tubing in the waste container.

4. Connect

Tube #2 from the 40 psi backpressure regulator that you connected in

step 2 on page 105 to the inlet port (In) on the pH valve.

5. Connect Tube #4 to the outlet port (Out) on the pH valve.

6. Connect the other end of Tube #4 to the Common port on the BioFrac fraction

collector diverter valve.

Tip: To clean the pH valve, immerse the outlet lines into a waste container and

the inlet lines into cleaning solution. Perform the system wash step in

Chr

omLab as explained in the section System CIP (Clean in Place) — Storage

Parameters in the NGC Chromatography Systems and ChromLab Software

User Guide.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 114