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Setting Delay Volume

Installation Guide | 9 3

Setting Delay Volume

The delay volume is the volume of fluid in the path between the UV or UV/Vis

detector and the fraction collector drophead. The system is typically plumbed with a

fixed length of tubing and a defined set of detectors (such as the conductivity

monitor and pH detector) between the UV or UV/Vis detector and the fraction

collector. The length of tubing and the volume of the flow cells between the UV or

UV/Vis detector and the drophead define the delay volume. Setting the delay

volume causes the fraction collector to wait for the sample to travel from the UV or

UV/Vis detector to the drophead before it starts to collect sample fractions.

In order to accurately predict which fraction collector tube or outlet container

contains the peak of inter

est, you have to account for the time it takes for the

volume of fluid to travel from the UV or UV/Vis detector to the container. Because

the tubing length and flow cells between the detector and the fraction collector

drophead do not change often, you can set the delay volume when you install

ChromLab. You can change the delay volume when you change the tubing length or

add other detectors along the flow path.

For more information about delay volume and event markers, see the online help or

the NGC Chr

omatography Systems and ChromLab Software User Guide.

To set the delay volume

1. Click File > System Settings to open the System Settings dialog box.

2. Choose the Delay Volume tab.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 95