Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 132)

5 | Plumbing and Priming the NGC Chromatography Systems

130 | NGC Chromatography Systems and ChromLab Software

3. To purge all lines of air bubbles and fill them with buffer:

a. Click All.

b. (Optional) Change the default flow rate.

The default flow rate is set to 20 ml/min.

c. Click Start.

The system pumps and the buffer blending valve start operating at the

specified flow rate, exchanging the solution in the inlet lines and expelling any

trapped bubbles, cycling sequentially thr

ough the ports Q1 to Q4. After the

specified duration, the pumps stop momentarily while the blending valve

switches to port Q3 in order to flush the lines with water before closing the

valve. No change is allowed during this process for the specified duration.

Note: You can stop the cycle before it completes by clicking Stop. In this

case, ensur

e that all buffer lines are filled with the appropriate buffers

before continuing.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 132