Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 136)

5 | Plumbing and Priming the NGC Chromatography Systems

134 | NGC Chromatography Systems and ChromLab Software

To prime the buffer inlet valves

1. Immerse the tubing for the inlet ports in their respective buffer containers.

2. On the NGC touch screen, open the System Pump dialog box.

3. In the System Pump dialog box, select the first inlet port to prime, for example

Buf

fer A 1.

4. Insert a 30 ml syringe into the priming port on system pump A.

5. Turn the priming port valve counter

-clockwise one full turn to open the seal.

6. Slowly withdraw the

plunger to withdraw liquid f

rom the port

until

air no

longer enters the syringe (approximately 10 ml) and all the tubing is filled

with liquid.

7. Turn the priming port valve clockwise to close the port.

8. Remove the syringe from the priming port and discard the liquid into waste.

1 2 ... 131 132 133 134 135 136 137 138 139 140 141 ... 177 178

Sem comentários

Bio-rad ChromLab™ Software Manual do Utilizador Página 136