Bio-rad ChromLab™ Software Manual do Utilizador descarregar pdf (Página 130)

5 | Plumbing and Priming the NGC Chromatography Systems

128 | NGC Chromatography Systems and ChromLab Software

5. Select Q4 as the port to open and prime.

6. Insert a 30 ml syringe into the priming port on system pump A.

7. Turn the priming port valve counter

-clockwise one full turn to open the seal.

8. Slowly withdraw the

plunger to withdraw liquid f

rom the port

until

air no

longer enters the syringe (approximately 10 ml)

and the tubing is filled with

liquid.

9. Turn the priming port valve clockwise to close the port.

10. Remove the syringe from the priming port and discard the liquid into waste.

11. Repeat steps 6–10 for ports Q3–Q1.

12. To prime system pump B, select port Q3 and perform steps 6–10.

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Bio-rad ChromLab™ Software Manual do Utilizador Página 130